CHEM 275 Introduction to Instrumental Analysis • 6 Cr.


Designed for students interested in a career in molecular biosciences. Topics include measurements and calculations in analytical chemistry, interaction of radiation with matter, spectroscopy, mass spectrometry, and separation techniques. Format includes lecture, discussion, and laboratory. Prerequisite: CHEM& 163, and BIOL& 160 or BIOL& 211. Recommended: ENGL& 235 and BTS 147.


After completing this class, students should be able to:

  • Record, analyze, report, and present scientific data.
  • Choose appropriate instrumentation or techniques based on sensitivity and precision required.
  • Calculate concentrations in solution, stoichiometric amounts, equilibrium constants, and other problem solving related to analytical chemistry.
  • Explain how molecular absorption of radiation is related to electronic, vibrational, and rotational transitions and how the process of emission of radiation occurs via fluorescence and phosphorescence.
  • Describe the major principles of spectroscopy, mass spectrometry, and separation techniques.
  • Identify and describe the major components of various types of analytical instrumentation, including spectroscopy instruments, mass spectrometers, and separation instruments.
  • Interpret spectroscopy data, mass spectra, and chromatography data for qualitative and quantitative analysis.